Lai Xianliang,Su Jia,Chen Ou.. The effect of CXCL12 on RANKL-induced osteoclastogenesis in RAW264.7 cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(3): 216-219,224.
Abstract：Objective: To investigate the effect of CXCL12 on receptor activator of NF-κB ligand (RANKL)
induced osteoclastogenesis in RAW264.7 cells. Methods: RAW264.7 cells were cultured in vitro and induced by RANKL. CXCL12 was used to stimulate cells at concentration of 0, 25, 50 and 100 ng/mL. The influence of CXCL12 on cell proliferation was assessed using cell counting kit-8 (CCK-8). RAW264.7 cells were induced by RANKL in control group, while 50 ng/mL CXCL12 was used in CXCL12 group and 650 nmol/L AMD3100 was used in AMD3100 group. Osteoclasts were determined with tartrate resistant acid phosphatase (TRAP) positive staining and their morphology was observed. The expression of MMP-9, c-src, Cathepsin K in mRNA was analyzed using RT-PCR. The protein expression of MMP-9, p-src, Cathepsin K was detected using Western blotting. Results: CXCL12 had no effect on the proliferation of RAW264.7 cells; TRAP staining positive number was significantly higher in the CXCL12 group (P<0.05) and lower in AMD3100 group (P<0.05); CXCL12 can promote the expression of mRNA in MMP-9, c-src, Cathepsin K and the protein of MMP-9, p-src, Cathepsin K in RANKL-induced cells (P<0.05). Conclusion: CXCL12 can promote the differentiation of RAW264.7 induced RANKL cells into osteoclasts and improve the bone resorption ability of the cells, which mean CXCL12 maybe a target for osteoporosis therapy.
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