LIN Yanyan,LIAO Tingting,JIN Jiaxi, et al. The suppressive effect of siILK on proliferation, migration and invasion of human extravillous trophoblastic cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(3): 168-171,177.
Abstract：Objective: To investigate the effects of integrin linked kinase (ILK) on the proliferation, migration and invasion of human extravillous trophoblastic cells (TEV-1). Methods: ILK-interferenced RNA was constructed and transfected into human TEV-1 cells, which was divided into experimental group (KD) and negative control group (NC); qRT-PCR and Western blot were used to detect the expression of ILK mRNA and protein. MTT assay, Transwell assay and Scarification test were used to detect the cell proliferation, migration and invasion. Results: Silenced ILK gene significantly reduced the ILK mRNA and protein expression levels of the TEV-1 cell (P<0.05), indicating that the transfection was successful. Silenced ILK gene significantly inhibited the proliferation, invasion (P<0.01) and migration of TEV-1 cells (P<0.05). Silenced ILK gene significantly decreased the mRNA and protein levels of MMP-9 and p-GSK3β in TEV-1 cells (P<0.01). Conclusion: These findings indicate that silenced ILK gene perturbs proliferation, migration and invasion may through down-regulated GSK3β and MMP-9 in TEV-1 cells.
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